The applicability of the foetal goat tongue cell line ZZ-R 127 to the isolation of foot-and-mouth disease virus (FMDV) has not been evaluated using clinical samples other than epithelial suspensions. Therefore, in the current study, ZZ-R 127 cells were used for the isolation of FMDV from clinical samples (e.g., sera, nasal swabs, saliva, faeces, and oropharyngeal fluids) collected from animals experimentally infected with an FMDV isolate. Virus isolation rates for the ZZ-R 127 cells were significantly higher than those for a porcine kidney cell line (IB-RS-2) in experimental infections using cattle, goats, and pigs (P < 0.01). Viral titres in the ZZ-R 127 cells were also significantly higher than those in the IB-RS-2 cells (P < 0.01). Thus, the applicability of ZZ-R 127 cells for the isolation of FMDV was confirmed for epithelial suspensions and other clinical samples.
(Exotic Diseases Research Division)
References:
Fukai K. et al (2013) J. Vet. Diag. Invest. 25(6):770-774