National Institute of Animal Health (NIAH)

Topics in Animal Health Research 1999

19. Production of Monoclonal Antibodies Against Bovine Kit Receptor

Japanese

Kit receptor is a receptor for stem cell factor (SCF). The extracellular domain of the bovine Kit receptor was produced by a baculovirus expression system. Six monoclonal antibody (mAb) clones were obtained from immunization of mice with the recombinant protein. Immunoprecipitaion and flow cytometric analysis indicated that all of the mAs specifically bound to the bovine Kit receptor expressed in COS-7 cells transfected with bovine Kit cDNA. Four of the six mAbs neutralized the biological activity of recombinant bovine SCF, whereas the other two did not. These mAbs would be valuable in studying possible bovine Kit-positive cell species such as hematopoietic cells, and in defining the biological role of the Kit receptor and its ligand, SCF, in cattle. (Lab. of Immunobiology, Department of Immunology TEL +81-298-38-7790)

References:

Hikono et al.: Production and characterization of monoclonal antibodies that recognize bovine Kit receptor. Vet. Immunol. Immunopathol., 68 : 101-112 (1999)

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