Vesicular stomatitis is an exotic disease for Japan. Vesicular stomatitis virus (VSV) infects wild animals as well as several domestic animals such a cattle, horses, and pigs. It has at least two major serotypes, the New Jersey serotype and the Indiana serotype. We developed a new molecular diagnostic assay which couples a PCR procedure to an enzyme-linked oligonucleotide sorbent assay (PCR-ELOSA). This PCR-ELOSA can not only detect viral RNA of both serotypes but also differentiate the two viral serotypes. The assay was shown to be more sensitive than the conventional RT-PCR method without cross-reaction.
(Research team for Exotic Diseases, TEL +81-29-838-7708)
