In the present study, we developed an allele-specific primer polymerase chain reaction (ASP-PCR) for genotyping a single nucleotide polymorphism (SNP) of swine toll-like receptor 5 (TLR5) (C1205T;P402L) that is related to the impaired recognition of Salmonella enterica serovar Choleraesuis (SC). The allele frequencies of this SNP were compared between several pig breeds in Japan and the Czech Republic. The swine TLR5 C1205T mutation was successfully determined by ASP-PCR using genomic DNA samples from Japan that had previously been genotyped by a sequencing method. Using the determined PCR conditions, genomic DNA samples from blood obtained from 110 pigs from seven different breeds from the Czech Republic were genotyped by this ASP-PCR method. The genotypes from the ASP-PCR completely matched those determined by the sequencing method. The allele frequency of the swine TLR5 C1205T mutation was 27.5% in the Landrace breed from the Czech Republic compared with 50.0% in Japanese Landrace breed. In Japan, the C1205T mutation was found only in the Landrace breed, whereas in the Czech Republic, it was found in both the Landrace and Pietrain breeds. These results indicate the usefulness of ASP-PCR for detecting a specific SNP for swine TLR5 affecting the ligand recognition. They also suggest the possibility of genetically improving pigs to enhance their resistance against SC infection by eliminating or selecting for this specific SNP of swine TLR5.
(Pathology and Pathophysiology Research Division)
References:
Muneta Y. et al. (2012) Microbiol. Immunol. 56:385-391
