To understand the interaction of E. rhusiopathiae with macrophages, we examined phagocytosis and intracellular killing of a virulent wild-type parent strain and acapsular avirulent transposon mutants by murine macrophages in vitro. In the presence of normal or immune serum, the parent and the mutant strains were both ingested; however, the number of ingested bacteria was three- to fourfold greater in the case of the mutant strains than in the case of the parent strain. The parent strain, but not the mutant strains, survived and replicated intracellularly when ingested in the presence of normal serum, whereas both the parent and the mutant strains were readily killed when ingested in the presence of immune serum. To further investigate the mechanism by which the parent strain can survive and replicate within macrophages, we studied the oxidative burst response of macrophages to these strains by measuring chemiluminescence (CL) and intracellular reduction of Nitro Blue Tetrazolium dye (NBT). Challenge exposure of macrophages with either the parent strain preopsonized with immune serum or the mutant strains preopsonized with normal or immune serum induced a strong oxidative burst, whereas the level was very low when the parent strain was preopsonized with normal serum. Phagocytosis of either the parent strain, in the presence of immune serum, or the mutant strains, in the presence of normal or immune serum, by macrophages reduced large amounts of intracellular NBT, whereas minimal amounts were reduced by the parent strain in the presence of normal serum. These results suggest that virulent E. rhusiopathiae can survive and subsequently replicate within murine macrophages when ingested in the presence of normal serum, and that the reduced production of reactive oxidative metabolites by macrophages may, in part, be responsible for this occurrence.
(Lab. of Physiologically Active Substances, Department of Biological Product)
