Canine monocytes and neurophils were cultured with chicken egg white derivatives (EWD) for 4 hours at 37ºC, and their phagocytic and chemotactic activities were evaluated by flow cytomertric analysis and a modified Boyden's chamber method, respectively. Significantly enhanced phagocytosis was shown in EWD-stimulated monocytes but not in EWD-stimulated neutrophils, Supplement of neutrophils with supermatant from monocytes cultured with EWD for 24 hours at 37ºC resulted in significantly increased phagocytosis. EWD was not chemotactic for neutrophils, However, significantly increased chemotactic activities of neutrophils were shown for the cluture supernatant of EWD-stimulated monocytes but not EWD-stimulated neutrophils. These results suggest that humoral factors produced by EWD-stimulated monocytes are involved in the enhancement of phagocytosis and chemotaxis of EWD-stimulated monocytes and neytrophils.
(Associate Director, Department of Immunology)
