Twenty monoclonal antibodies (MAbs) were produced against the virulent ALD or vaccinal GPE- strains of classical swine fever (CSF) virus. The MAbs were divided into 7 groups on the basis of the difference in the reaction spectrum to various CSF and bovine viral diarrhea-mucosal disease (BVD-MD) viruses. The panel of the MAbs could classify 20 CSF viruses into 6 types, and could discriminate between not only CSF and BVD-MD viruses but also the vaccinal GPE- strain and the field isolates of CSF virus. The results indicate that the MAbs are useful for diagnosis and investigation of CSF viruses.
(Lab. of Clinical Microbiology, Hokkaido Research Station)
