Genetic diversity of porcine teschoviruses (PTVs; renamed from porcine enterovirus 1; PEV-1) and porcine enteroviruses (PEVs) was studied. The nucleotide and derived amino acid sequences from the RNA-dependent RNA polymerase (RdRp) region, the capsid VP2 region and the 3' non-translated region (3'NTR) were studied, and compared with other picornaviruses. The data were obtained by sequencing PCR amplicons and 3'RACE products using the primers based on the sequences of PTV (porcine teschovirus) and the available PEV (porcine enterovirus) strains. To construct the PTV primers, the majority of the genomic sequence of the PTV reference strain Talfan was determined. Phylogenetic analysis of RdRp/VP2 and the secondary structure analysis of 3'NTR indicated that PEVs should be re-classified genetically into three groups: One group that should be assigned to PTVs and two PEV subspecies represented by PEV-8 V13 strain and PEV-9 UKG410/73 strain. (Lab. of Pathogenic Viruses, Department of Virology TEL +81-298-38-7763)
