National Institute of Animal Health (NIAH)

Topics in Animal Health Research 2000

17. The Effect of Cellular Triglyceride Accumulation on Apolipoprotein B-100 Secretion From Bovine Hepatocytes


  To investigate the relationship between apolipoprotein B-100 (ApoB-100) secretion and triglyceride (TG) accumulation in liver parenchymal cells, the effects of carnitine and propionate on ApoB-100 secretion and TG accumulation in primary cultured bovine hepatocytes were examined. In the presence of oleic acid (0.5 - 2 mM), carnitine (1 mM) increased ApoB-100 secretion and decreased TG accumulation in cells. Propionate (5 mM) decreased ApoB-100 secretion and increased TG accumulation in cells. In the absence of oleic acid, neither carnitine nor propionate had no significant effect on Apo B-100 secretion. These results suggested that ApoB-100 secretion from bovine hepatocyte was dependent on TG content in the cells. (Metabolic Disease Section, Department of Production Diseases TEL+81-298-38-7795)