National Institute of Animal Health (NIAH)

Topics in Animal Health Research 2003

06. Monoclonal antibody-based immunohistochemical diagnosis of Nipah virus infection


   The immunohistochemical reactivity of mouse monoclonal and rabbit polyclonal antibodies raised against Nipah virus antigens were investigated using formalin-fixed, paraffin embedded porcine and equine lung tissues from experimental Nipah and Hendra virus infections, respectively. Lung tissues of each animal were sampled at 7 days post-inoculation and fixed in 10% neutral-buffered formalin for 1 week. Either microwave irradiation or enzymatic digestion effectively unmasked the viral antigens in formalin-fixed, paraffin-embedded tissue sections. Two clones of monoclonal antibodies (11F6 and 13A5) reacted with Nipah virus-infected porcine lung tissue, but not with Hendra virus-infected equine lung tissue. Rabbit polyclonal antibodies reacted with both Nipah and Hendra viral antigens. These Nipah virus-specific monoclonal antibodies and cross-reactive polyclonal antibodies may therefore be useful for immunohistochemical differential diagnosis of Nipah and Hendra virus infections.
(Diagnostic Section, Department of Epidemiology   TEL +81-29-838-7774)


  • Imada et al. (2004) J. Vet. Med. Sci. 66:81-83.
  • Tanimura et al.(2004) J.Comp.Path.131:199-206.
  • Tanimura et al.(2004) J.Vet.Med.Sci. 66:1263-1266.