The immunohistochemical reactivity of mouse monoclonal and rabbit polyclonal antibodies raised against Nipah virus antigens were investigated using formalin-fixed, paraffin embedded porcine and equine lung tissues from experimental Nipah and Hendra virus infections, respectively. Lung tissues of each animal were sampled at 7 days post-inoculation and fixed in 10% neutral-buffered formalin for 1 week. Either microwave irradiation or enzymatic digestion effectively unmasked the viral antigens in formalin-fixed, paraffin-embedded tissue sections. Two clones of monoclonal antibodies (11F6 and 13A5) reacted with Nipah virus-infected porcine lung tissue, but not with Hendra virus-infected equine lung tissue. Rabbit polyclonal antibodies reacted with both Nipah and Hendra viral antigens. These Nipah virus-specific monoclonal antibodies and cross-reactive polyclonal antibodies may therefore be useful for immunohistochemical differential diagnosis of Nipah and Hendra virus infections.
(Diagnostic Section, Department of Epidemiology TEL +81-29-838-7774)
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