National Institute of Animal Health (NIAH)

Topics in Animal Health Research 2004

06. Characterization of the 1,297 Strains of Erysipelothrix Isolated from Pigs Affected with Erysipelas during the Last 17 Years by Serotype and Genotype


One thousand two hundred ninety-seven Erysipelothrix strains isolated from swine erysipelas cases between 1988 and 2004 in Japan were characterized by serotype, genotype, and acriflavine resistance. Thirty-eight, 67, 80 and 1,112 strains were isolated from acute septicemia, urticaria, chronic endocarditis and chronic arthritis cases, of which 635 isolates belonged to serotype 1a. Of the serotype 1a isolates, 75% were acriflavine-resistant and 81% showed a live vaccine strain-characteristic 253 bp band in randomly amplified polymorphic DNA analysis (RAPD), designated RAPD type 1-2. Almost all of these acriflavine-resistant strains or RAPD type 1-2 strains were isolated from cases of chronic arthritis. The incidence of acriflavine-resistant strains in the RAPD type 1-2 was markedly higher than in other RAPD types and serotypes. Furthermore, the mouse pathogenicity of the RAPD type 1-2 was lower than in other RAPD types of serotype 1a. Our results indicate that the RAPD typing is a useful method for the discrimination of avirulent strains of serotype 1a from virulent strains of serotype 1a.
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Imada et al. (2004) J. Clin. Microbiol. 42:2121-2126.