National Institute of Animal Health (NIAH)

Topics in Animal Health Research 2006

01. Development of a mass production system for recombinant porcine lysozyme

Japanese

  To establish a mass-production system for the porcine lysozyme (PLY), a powerful candidate to replace antibiotics, several kinds of expression systems and conditions were examined. PLY gene (length: 484bp) was synthesized from nine short DNA oligomers by the SPR method and PCR extension. This artificial gene was expressed in two kinds of yeast cell lines (kk-4, A2-1-1A) and in three kinds of insect cell lines (BmN4, Sf-9, expresSF+). Among them, the expresSF+ cells were more efficient (more than 50mg/L) than other cells. Then, PLY was produced by expresSF+ cells using a culture media under different osmotic pressures (194 − 321mOsm/kg). The results indicated that a low osmotic pressure (220 − 309mOsm/kg) was the most efficacious. Especially, the culture medium with 296mOsm/kg of osmotic pressure had the highest productivity for recombinant PLY, and reached about 80mg/L. This system has applied for a patent (patent application number: 2007-025410).
(Research Team for Advanced Biologicals, +81-42-321-1441)

Centers・Institutes