National Institute of Animal Health (NIAH)

Topics in Animal Health Research 2007

12. Differentiation of foot-and-mouth disease virus-infected pigs from vaccinated pigs using a western blotting assay based on baculovirus-expressed nonstructural proteins 2C and 3D

Japanese

  Baculovirus-expressed foot-and-mouth disease virus (FMDV) nonstructural proteins 2C and 3D were used as the antigens in a western blotting assay. This assay allowed the differentiation of FMDV-infected pigs from vaccinated pigs. Serial studies were performed using sera collected from experimentally infected and vaccinated pigs. Positive reactions were found in the sera derived from experimentally infected pigs but no positive reaction was found in vaccinated pigs. These results suggest that this western blotting assay is a useful method for the differentiation of infected pigs from vaccinated pigs.
(Research team for Exotic Diseases, TEL +81-29-838-7708)

Reference:

  • Fukai et al. (2007) 13th international world association of veterinary laboratory diagnosticians symposium.
  • Yamazoe et al. (2007) The 55th annual meeting of the Japanese society for virology.

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