We developed a monoclonal antibody (MAb)-based sandwich direct enzyme-linked immunosorbent assay (MSD-ELISA) for detecting foot-and-mouth disease virus (FMDV) type O antigens. The specificity and sensitivity of the MSD-ELISA was improved by using a type O virus-neutralizing MAb. In this study, clinical samples such as saliva and plasma of type O FMDV-inoculated pigs were examined using MSD-ELISA and the standard method of indirect sandwich (IS) ELISA. MSDELISA revealed type O antigens in both samples (saliva and plasma) with higher sensitivity than ISELISA. In general, viremia tends to appear earlier than the clinical signs in FMD. Therefore, it is possible to use the MSD-ELISA for antigen detection at the preclinical or subclinical stage of a FMDV infection by using plasma and/or saliva.
(Research Team for Exotic Diseases, TEL +81-29-838-7708)
Reference:
Morioka, K. et al. (2009) J. Clin. Microbiol. 47:3663-3668.
