We performed molecular-based identification of yeasts that were associated with bovine clinical mastitis in Japan. We examined over 3200 quarter milk samples that were collected from Holstein dairy cows in 2011 on the islands of Hokkaido and Honshu. Yeast isolates were characterised by polymerase chain reaction amplification and sequencing of the D1/D2 region of the 26S rDNA gene. Molecular characterisation confirmed that Candida spp. and Pichia spp. were the most frequently isolated species. Our molecular analysis of mastitic milk samples demonstrated the prevalence of Pichia kudriavzevii (22/58) and Candida tropicalis (14/58). In addition, we demonstrated that molecular analysis of the D1/D2 region of the 26S rDNA gene is a rapid and reliable method for identifying clinically significant yeasts for dairy hygiene, including potentially new or emerging pathogenic species.
(Dairy Hygiene Research Division)
Hayashi T. et al. (2013) J. Vet. Med. Sci. 75:387-390