National Institute of Animal Health (NIAH)

Topics in Animal Health Research 2014

07. The first isolation of genotype C bovine parainfluenza virus type 3 in Japan


Bovine parainfluenza virus type 3 (BPIV3) causes severe respiratory illness in cattle associated with bovine respiratory disease complex worldwide. BPIV3 viruses are classified into three genotypes (BPIV3a-c), and all the Japanese strains published to date have been included in BPIV3a. In the present study, we first isolated BPIV3c from the nasal swab of a cow with respiratory symptoms in Japan. In addition, we determined the complete genome sequence of the BPIV3c isolate HS9. The genome sequence of the HS9 isolate showed low nucleotide sequence similarity to those of the Japanese BPIV3a strains, suggesting that the current RT-PCR method using BPIV3a-specific primers will fail to detect BPIV3c strains. Further sequence analysis of BPIV3 strains and development of an assay to detect all genotypes of BPIV3 will be required for the accurate diagnosis of BPIV3 infection.
(Viral Diseases and Epidemiology Research Division)


Konishi M. et al (2014) Genome Announc. 2(6) e01215-14. (doi:10.1128/genomeA.01215-14)