National Institute of Animal Health (NIAH)

Topics in Animal Health Research 2014

31. Complete genome sequencing of two Mycoplasma species causing bovine mastitis


Mycoplasmal bovine mastitis features strong infectivity, severe symptoms, and poor response to treatment with antibiotics. Therefore, this disease incurs huge economic losses. Although causal species of this disease in Japan had been Mycoplasma bovis and M. bovigenitalium for many years, M. californicum and M. canadense have often been isolated from mastitis cases since 2005. For these species, much information, including genetic information or etiologic factors, are unexplained. In this study, we completed the first annotated genome sequence of M. californicum str. HAZ160_1 and M. canadense str. HAZ360_1, which were isolated from bovine mastitis in Japan. Both genomes consist of a single circular chromosome of 799,088 bp and 693,241 with an average GC content of 30.8% and 24.34%, respectively. The chromosomes contain 574 and 484 protein-coding genes, 31 and 32 transfer RNA genes for all amino acids, and two sets of rrn operons, respectively. Although the genome sequence of M. californicum contained genes of proteins involved in the synthesis of capsular polysaccharide and the antigenic variation shifting surface proteins (Vsps), which is major etiologic factors, we confirmed only the genes of Vsps in the M. canadense genome. These whole-genome sequences have been registered at DDBJ/EMBL/GenBank under accession nos. AP013353 and AP014631.
(Dairy Hygiene Research Division)


  • Hata E. and Murakami K. (2014) Genome Announc. 2(4):e00684-14 (doi:10.1128/genomeA.00684-14)
  • Hata E. (2014) Genome Announc. 2(5):e00984-14 (doi:10.1128/genomeA.00984-14)