To reliably detect early pregnancy factor (EPF) in cattle, monoclonal antibody specific for bovine CD2 molecule was applied to the rosette inhibition test. The results obtained have indicated that anti-bovine CD2 monoclonal antibody can be utilized with the rosette inhibition test to detect EPF in cattle, and that this assay detects bovine EPF for pregnancy serum at least 24 hours after ovulation.
(Lab. of Theriogenology, Department of Pathology and Physiology)
