National Institute of Animal Health (NIAH)

Topics in Animal Health Research 1998

09. Mapping the Genetic Determinants of Pathogenicity in Swine Vesicular Disease Virus

Japanese

A series of recombinant viruses were constructed using infectious cDNA clones of the virulent J1'73, and the avirulent H/3'76 strains of swine vesicular disease virus to identify the genetic determinants of pathogenicity. These determinants could be mapped to the region between nucleotides (nt) 2233 and 3368 corresponding to the C terminus of VP3, the whole of VP1 and the N terminus of 2A. In this region, there are eight nucleotide differences leading to amino acid changes between the two strains. To map more precisely the determinants of pathogenicity, site-directed mutagenesis was performed to produce the mutant viruses. From the results of animal experiments with two mutant viruses, we concluded that two amino acids (VP1-132 and 2A-20), which were shown as plaque size determinants in the last study, are also genetic determinants of pathogenicity.
(Lab of Diagnosis, Department of Exotic Disease, TEL +81-42-321-1441)

Reference:

Kanno, T., et al. :1999. Mapping the genetic determinants of pathogenicity and plaque phenotype in swine vesicular disease virus. J. Virol., 73 : 2710-2716

Centers・Institutes