National Institute of Animal Health (NIAH)

Topics in Animal Health Research 1999

01. Detection of Porcine Coronavirus from Clinical Specimens by Immunofluorescent Assay (IFA) and RT-PCR

Japanese

The rapid differential diagnosis of the Porcine epidemic diarrhea virus (PEDV) and the Transmissible gastroenteritis virus (TGEV) was achieved by RT-PCR from clinical specimens of pigs having diarrhea. The PEDV antigen was detected by IFA using a PEDV-specific monoclonal antibody on infected cells. These rapid and specific methods were available for diagnosis of pig diarrhea. (Lab. of Clinical Virology, Kyushu Research Station TEL +81-99-268-2078)

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