National Institute of Animal Health (NIAH)

Topics in Animal Health Research 2000

24. Characteristics of an Isolated Foot-and-Mouth Disease Virus in Japan

Japanese

  In Japan four cases of Foot-and-Mouth disease (FMD) occurred from March to May 2000. The FMD virus (FMDV) was isolated from two probang samples of Japanese black cattle in the third case in Miyazaki prefecture. These Japanese black cattle showed clinical signs of salivation, erosion, ulcers in the mouth and nose without forming any vesicular. In order to isolate the virus, the probang samples were inoculated to bovine kidney cells (BK) and bovine thyroid cells (BTh). CPE was observed in the primary BK cells two days after post-inoculation. Specific amplified DNA segments for FMDV were detected by reverse transcriptase polymerase chain reaction (RT-PCR) from the virus culture fluid. The virus was identified as FMDV type O by ELISA for antigen detection. The nucleotide sequence encoding VP1 was also determined. It shows that the sequence was the same as that of the formally detected PCR product in the first case, in which the virus could not be isolated. It was shown that this FMDV is one of the strains which is wide-spread in Asia and this virus strain was named O/JPN/2000 by the Pirbright Institute in England, the World Reference Laboratory for FMD. (Diagnosis Section, Department of Exotic Diseases TEL;81-42-321-1441)

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