In this paper, we describe the production of anti-porcine interleukin-18 (IL-18) monoclonal antibodies (mAbs). Fifteen anti-porcine IL-18 mAbs were established and characterized. They were all useful for the immunoblot analysis of porcine IL-18. A sandwich enzyme-linked immunosorbent assay (ELISA) was developed using two different mAbs (7-G-8 and 5-C-5). Porcine IL-18 was quantified with a detection limit of 20 pg/ml using this ELISA. Four (2-C-4, 5-F-6, 9-H-6, and 12-C-12) of fifteen mAbs were shown to be useful for immunohistochemical staining of porcine IL-18 and detected porcine IL-18 in Kupffer cells and macrophages in hepatic focal necrosis of piglets with experimental endotoxemia induced by E.coli lipopolysaccharide. Furthermore, porcine IL-18 was obtained easily from the supernatant of insect cells containing porcine IL-18 by immunoaffinity chromatography using an anti-porcine IL-18 mAb (2-2-B). The anti-porcine IL-18 mAbs developed in this study, which are useful for the immunoblotting, sandwich-ELISA, immunohistochemical staining, and purification for porcine IL-18, will become very powerful tools for investigating the role of porcine IL-18 in various immune responses and diseases of pig. (Immune System Section, Department of Immunology TEL +81-298-38-7857)
Reference:
1.Muneta et al. (2000) J. Immunol. Methods 236:99-104.
2.Muneta et al. (2000) J. Interferon Cytokine Res. 20:331-336.
