National Institute of Animal Health (NIAH)

Topics in Animal Health Research 2004

03. Pig ascariasis vaccine by mucosal route


We evaluated the vaccinal effects of As16 in pigs, the natural host of A. suum, using the intranasal route of immunization. Pigs that received E. coli-expressed recombinant As16 coupled with cholera toxin (rAs16-CT) had significantly elevated levels of rAs16-specific serum IgG and mucosal-associated IgA antibodies. The recombinant protein evoked a type II immune response characterized by elevated levels of IL-4 and IL-10 in the culture supernatants of the peripheral blood mononuclear cells of the vaccinated pigs. An increased level of rAs16-specific serum IgG1 was also detected. Pigs vaccinated with rAs16-CT were protected from challenged A. suum larval migration through the lungs, as indicated by a 58% reduction in the recovery of lung-stage L3 compared with that in the non-vaccinated controls. Purified-Ig from rAs16-CT-vaccinated pigs inhibited the survival of infective L3, and interrupted the molting of lung-stage L3. We conclude that rAs16 is a promising mucosal vaccine candidate for pig ascariasis.
(Parasitic Disease Section, Department of Infectious Diseases TEL +81-29-838-7751)


  • Tsuji et al. (2003) Infect. Immun. 71:5314-5322.
  • Tsuji et al. (2004) J. Infect. Dis. 190:1812-1820.