National Institute of Animal Health (NIAH)

Topics in Animal Health Research 2009

19. Interleukin-10 assay with recombinant PPE antigen of Mycobacterium avium subsp. paratuberculosis for the diagnosis of Johne's disease


  The recombinant Mycobacterium avium subsp. paratuberculosis (Map) PPE protein, Map41, which has been reported as one of the interferon-gamma (IFN-γ)-inducing antigens of Map, also significantly induced the secretion of interleukin-10 (IL-10) from blood cells obtained from infected calves. The elicited IL-10 production in response to Map41 from experimentally infected calves was detectable as early as 2 weeks after inoculation with Map. In addition, the IL-10 production was detected earlier than IFN-γ production. Furthermore, the induction of IL-10 also showed high specificity to Map in calves and guinea pigs experimentally infected with various Mycobacterium species. These observations suggest that an IL-10 assay is a useful diagnostic test in the early stage of Johne's disease.
(Research Team for Paratuberculosis, TEL +81-29-838-7708)


Nagata, R. et al. (2010) Vet. Immunol. Immunopathol. 135:71-78.