National Institute of Animal Health (NIAH)

Topics in Animal Health Research 2009

25. Development of a bovine hepatocytes culture technique to study dioxin-like effects

Japanese

  This study investigated the expression of CYP1A1 and CYP1B1 in primary cultured bovine hepatocytes exposed to several dioxins, furans, and animal feed ingredients to identify their dioxinlike potentials. Hepatocytes were isolated from calf liver, cultured, and treated for 24 h with the target compounds or extracts. Quantitative real-time polymerase chain reaction analysis (qRT-PCR) showed that the relative mRNA levels for CYP1A1 and CYP1B1 exhibited a dose-dependent induction by 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD). The EC50 of TCDD for the CYP1A1 expression was approximately 4-fold less than that of CYP1B1. 2,3,7,8-tetrabromodibenzo-p-dioxin (TBDD) had the highest dioxin-like toxic potential for tested PBDD/Fs. A good correlation was also observed in highresolution gas chromatography/high resolution mass spectrometry (HRGC/HRMS)-derived dioxin-like toxic equivalents (TEQs) in fish oil samples and relative CYP1A1 mRNA induction in bovine hepatocytes treated with purified fish oil extracts. These data suggested that quantification of biomarker regulations in primary cultured hepatocytes could represent an effective tool for both the screening and study of various chemical entities in large animals.
(Safety Research Team, TEL +81-29-838-7708)

Reference:

Guruge, K. S. et al. (2009) Toxicology Letters. 185(3):193-196.

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