The recombinant Newcastle disease virus (rNDV) vector has been used as a live attenuated vaccine vector to construct the avian influenza (AI) vaccine in China and Mexico. The rNDV vector-based AI vaccine induces effective protection against infection by avian influenza virus (AIV). However, the efficacy of the rNDV vector vaccine deteriorates when the chickens are vaccinated against Newcastle disease virus (NDV). In this study, we investigated the candidate strains from avian paramyxoviruses (APMV) of different subtypes to overcome this problem.
We focused on the avian paramyxoviruses (APMVs) as a vaccine vector candidate because they replicate in the respiratory and digestive tracts of chickens as does AIV, and are expected to induce mucosal immunity. APMV serotypes 2 to 10 were examined for their cross-reactivities against anti-NDV antisera and for replication in chickens immunized against NDV. APMV serotypes 2, 6 and 10 showed low cross-reactivities to anti-NDV antisera by the haemagglutination inhibition and virus neutralization tests. They replicated in the respiratory tract and induced antibody responses in 7-week-old chickens immunized against NDV. These results suggested that APMV serotypes 2, 6 and 10 could be promising candidates as vaccine vectors for chickens.
(Influenza and Prion Disease Research Division)
References:
Tsunekuni R. et al (2014) Vet Immunol Immunopathol. 160(3-4):184-91
