It is intended to provide a method of reducing the total amount of stored protein、 to develop a technique required therefor、and to provide a plant and its seeds developed by the above method and a method of using such a plant and seeds. More specifically、 a nucleic acid molecule containing a consecutive nucleic acid sequence of at least 15 in length which is complementary with a nucleic acid sequence encoding prolamin polypeptide or a nucleic acid sequence having a homology of at least about 70% to the complementary nucleic acid sequence having at least 15 nucleotide length. A method of reducing the expression dose of a protein in a seed of a plant which comprises: A) the step of providing the above-described nucleic acid molecule; B) the step of transferring the nucleic acid molecule into the cells of the plant; C) the step of re-differentiating the cells to construct a transgenic plant; and D) the step of obtaining seeds from the transgenic plant.
出願日 | 出願番号 | 公開日 | 公開番号 | 登録日 | 登録番号 |
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2005年5月31日 | 2004-562037 | 2004年7月 8日 | WO/2004/056993 | 2010年12月17日 | 4644777 |
出願人 | |||||
機構内発明者 | |||||
黒田 昌治 |