食品研究部門

食品醸造微生物ユニット

主要成果

  • 楠本憲一、曲山幸生、発酵食品データベースの構築、日本食品科学工学会誌、第66巻第3号、74-82 (2019) doi : 10.3136/nskkk.66.74
  • Metal Ions in Activated Carbon Improve the Detection Efficiency of Aflatoxin-Producing Fungi. Tadahiro Suzuki*, Masatoshi Toyoda, Toxins, 11(3), 140, 1-13 (2019)
  • Gamma-glutamylcysteine synthetase gene homolog (gshA) is important in glutathione homeostasis in Aspergillus oryzae, Ryota Hattori, Sawaki Tada, Mayumi Matsushita-Morita, Satoshi Suzuki and Ken-Ichi Kusumoto*, Japan Agricultural Research Quarterly. 52(4), 301-305 (2018)
  • Aspergillus oryzae Strain with Improved Conidiation after Light Stimulation, Pushpa S. Murthy, Motoaki Sano, Ryota Hattori, Ken-Ichi Kusumoto, Satoshi Suzuki, Japan Agricultural Research Quarterly. 52(1), 23-28 (2018)
  • Light-IrradiationWavelength and Intensity Changes Influence Aflatoxin Synthesis in Fungi. Tadahiro Suzuki, Toxins, 10(1), 31, 1-12 (2018)
  • Enzymatic characterization of a novel Xaa-Pro aminopeptidase XpmA from Aspergillus oryzae expressed in Escherichia coli. Matsushita-Morita M, Tada S, Suzuki S, Hattori R, Kusumoto KI, Journal of bioscience and bioengineering, 124 (5), 534-541 (2017)
  • Wide target analysis of acylglycerols in miso (Japanese fermented soybean paste) by supercritical fluid chromatography coupled with triple quadrupole mass spectrometry and the analysis of the correlation between taste and both acylglycerols and free fatty acids. Ogawa T, Izumi Y, Kusumoto K, Fukusaki E, Bamba T, Rapid communications in mass spectrometry. 31(11) 928-936 (2017)
  • Addition of Carbon to the Culture Medium Improves the Detection Efficiency of Aflatoxin Synthetic Fungi. Tadahiro Suzuki*, Yumiko Iwahashi, Toxins, 8(11), 338, 1-15 (2016)
  • A novel non-thermostable deuterolysin from Aspergillus oryzae. Maeda H, Katase T, Sakai D, Takeuchi M, Kusumoto K, Amano H, Ishida H, Abe K, Yamagata Y, Bioscience, biotechnology, and biochemistry. 80(9) 1813-1819 (2016)
  • Three extracellular dipeptidyl peptidases found in Aspergillus oryzae show varying substrate specificities. Maeda H, Sakai D, Kobayashi T, Morita H, Okamoto A, Takeuchi M, Kusumoto K, Amano H, Ishida H, Yamagata Y. Appl Microbiol Biotechnol. 100(11) 4947-4958. (2016)
  • Acid protease production by Aspergillus oryzae on potato pulp powder with emphasis on glycine releasing activity: a benefit to the food industry, P. S. Murthy and K.-I. Kusumoto*, Food and Bioproducts Processing, 96, 180-188 (2015) doi:10.1016/j.fbp.2015.07.013
  • Effect of light on the growth and acid protease production of Aspergillus oryzae. Pushpa S. MURTHY, Satoshi SUZUKI and Ken-Ichi KUSUMOTO*, P. S. Murthy, S. Suzuki and K.-I. Kusumoto*, Food Science and Technology Research, 21 (4), 631-635 (2015) doi: 10.3136/fstr.21.631
  • Culture-independent bacterial community analysis of the salty-fermented fish paste products of Thailand and Laos. Marui J, Boulom S, Panthavee W, Momma M, Kusumoto K, Nakahara K, Saito M, Biosci Microbiota Food Health. 34(2):45-52. (2015) doi: 10.12938/bmfh.2014-018.
  • Telomere-mediated chromosomal truncation in Aspergillus oryzae. Tada S, Ohkuchi H, Matsushita-Morita M, Furukawa I, Hattori R, Suzuki S, Kashiwagi Y, Kusumoto K-I. Journal of Bioscience and Bioengineering, 119 (1), 43-46 (2015) (doi: 10.1016/j.jbiosc.2014.06.011.
  • Culture-independent analysis of the bacterial community during fermentation of pa-som, a traditional fermented fish product in Laos. Junichiro Marui, Sayvisene Boulom, Wanchai Panthavee, Mari Momma, Ken-Ichi Kusumoto, Kazuhiko Nakahara, Masayoshi Saito. Fisheries Science. Fish. Sci. 80(5), 1109-1115 (2014)
  • Characterization of acid phosphatase (AphC) from miso koji mold, Aspergillus oryzae KBN630, AphC is mainly responsible for both acid phosphatase activity and 5'-IMP dephosphorylation activity in soybean-koji culture, S Yoshino-Yasuda, E Nakamura, N Ono, O Hasegawa, Y Iga, Y Shiraishi, Y Wagu, K Sugimoto, T Suzuki, T Sugimoto, K-I Kusumoto, M Kato and N Kitamoto, Food Sci. Technol. Res. 20(2),367-374 (2014)
  • conF and conJ contribute to conidia germination and stress response in the filamentous fungus Aspergillus nidulans, Suzuki S, Sarikaya Bayram Ö, Bayram Ö, Braus GH, Fungal Genet Biol. 2013 Jul;56:42-53. doi: 10.1016/j.fgb.2013.04.008.
  • Characterization of a D-Stereoselective Aminopeptidase (DamA) Exhibiting Aminolytic Activity and Halophilicity from Aspergillus oryzae. Matsushita-Morita M, Nakagawa H, Tada S, Marui J, Hattori R, Suzuki S, Yamagata Y, Amano H, Ishida H, Takeuchi M, Kusumoto KI. Appl Biochem Biotechnol. 171(1):145-164 (2013) doi: 10.1007/s12010-013-0330-z.
  • Reduction of the degradation activity of umami-enhancing purinic ribonucleotide supplement in miso by the targeted suppression of acid phosphatases in the Aspergillus oryzae starter culture. Marui J, Tada S, Fukuoka M, Wagu Y, Shiraishi Y, Kitamoto N, Sugimoto T, Hattori R, Suzuki S, Kusumoto K. International Journal of Food Microbiology, 166(2):238-243 (2013) doi: 10.1016/j.ijfoodmicro.2013.07.006.
  • A serine-type carboxypeptidase KexA of Aspergillus oryzae has broader substrate specificity than yeast Kex1, and is required for normal hyphal growth and conidiation. Morita H, Tomita S, Maeda H, Okamoto A, Yamagata Y, Kusumoto KI, Amano H, Ishida H, Takeuchi M. Appl Environ Microbiol. 78(22):8154-8157 (2012)
  • Comparison of Acid Phosphatase Gene Expression Profiles in Solid-State Rice and Soybean Cultures of an Aspergillus oryzae Strain with Low Acid Phosphatase Activity (KBN8048): Implications for Miso Brewing. Marui J, Tada S, Fukuoka M, Suzuki S, Hattori R, Wagu Y, Shiraishi Y, Kitamoto N, Sugimoto T, and Kusumoto K., Food Sci. Technol. Res. 18(1),83-90 (2012)
  • Disruption and overexpression of acid phosphatase gene (aphA) from a miso koji mold, Aspergillus oryzae KBN630, and characterization of the gene product, S. Yoshino-Yasuda, O. Hasegawa, Y. Iga, Y. Shiraishi, Y. Wagu, T. Suzuki, T. Sugimoto, K.-I. Kusumoto, M. Kato, and N. Kitamoto, Food Sci. Technol. Res. 18(1),59-65 (2012)
  • Enzymatic properties of the glycine D-alanine aminopeptidase of Aspergillus oryzae and its activity profiles in liquid-cultured mycelia and solid-state rice culture (rice-koji). Marui J, Matsushita-Morita M, Tada S, Hattori R, Suzuki S, Amano H, Ishida H, Yamagata Y, Takeuchi M, Kusumoto K., Appl. Microbiol. Biotechnol. 93, 655-669 (2012)
  • Genomic Analysis of Koji Mold Aspergillus oryzae and Investigation of Novel Peptidases by Post-genomic Approach. Kusumoto K., Japan Agricultural Research Quarterly. 46(1), 1-6 (2012)
  • Morita H, Abo H, Okamoto A, Maeda H, Yamagata Y, Kusumoto K, Amano H, Ishida H, Takeuchi M.: Enzymatic properties of the recombinant serine-type carboxypeptidase OcpC, which is unique to Aspergillus oryzae. Biosci Biotechnol Biochem., 75(4), 662-8 (2011).
  • Hattori R, Matsushita-Morita M, Marui J, Tada S, Suzuki S, Furukawa I, Yamagata Y, Amano H, Ishida H, Takeuchi M, Kusumoto K.: Characterization of an Aspergillus oryzae cysteinyl dipeptidase expressed in Escherichia coli. Biosci Biotechnol Biochem., 75(1), 159-61 (2011).
  • Matsushita-Morita M, Tada S, Suzuki S, Hattori R, Marui J, Furukawa I, Yamagata Y, Amano H, Ishida H, Takeuchi M, Kashiwagi Y, Kusumoto K.: Overexpression and characterization of an extracellular leucine aminopeptidase from Aspergillus oryzae. Curr Microbiol., 62(2), 557-64 (2011).
  • Morita H, Kuriyama K, Akiyama N, Okamoto A, Yamagata Y, Kusumoto K, Koide Y, Ishida H, Takeuchi M.: Molecular cloning of ocpO encoding carboxypeptidase O of Aspergillus oryzae IAM2640. Biosci Biotechnol Biochem., 74(5), 1000-6 (2010).
  • Suzuki S, Fukuoka M, Ookuchi H, Sano M, Ozeki K, Nagayoshi E, Takii Y, Matsushita M, Tada S, Kusumoto K, Kashiwagi Y.: Characterization of Aspergillus oryzae glycoside hydrolase family 43 beta-xylosidase expressed in Escherichia coli. J Biosci Bioeng., 109(2), 115-7 (2010).
  • Matsushita-Morita M, Furukawa I, Suzuki S, Yamagata Y, Koide Y, Ishida H, Takeuchi M, Kashiwagi Y, Kusumoto KI.: Characterization of recombinant prolyl aminopeptidase from Aspergillus oryzae. J Appl Microbiol., 109(1), 156-65 (2010).
  • Kumagai, S. Tada, K. Nozaki, M. Mizuno, T. Kanda, S. Suzuki, K.-I. Kusumoto, T. Sasaki, Y. Kashiwagi, Y. Amano: Enzymatic production of Glucosylxylose using a cellobiose phosphorylase-yeast combined system. J. Appl. Glycosci., 58, 31-34 (2010).
  • S. Suzuki, M. Fukuoka, S. Tada, M. Matsushita-Morita, R. Hattori, N. Kitamoto, K.-I. Kusumoto.: Production of polygalacturonase by recombinant aspergillus oryzae in solid-state fermentation using potato pulp. Food Sci. Technol. Res., 16(5), 517-521 (2010)
  • Tada S, Matsushita-Morita M, Suzuki S, Kusumoto K, Kashiwagi Y.: Characterization of a neutral ceramidase orthologue from Aspergillus oryzae. FEMS Microbiol Lett., 298(2), 157-65 (2009).
  • Morita H, Okamoto A, Yamagata Y, Kusumoto K, Koide Y, Ishida H, Takeuchi M.: Heterologous expression and characterization of CpI, OcpA, and novel serine-type carboxypeptidase OcpB from Aspergillus oryzae. Appl Microbiol Biotechnol., 85(2), 335-46 (2009).
  • Matsushita M, Tada S, Suzuki S, Kusumoto K, Kashiwagi Y.: Deletion analysis of the promoter of Aspergillus oryzae gene encoding heat shock protein 30. J Biosci Bioeng., 107(4), 345-51 (2009).
  • Suzuki S, Tada S, Fukuoka M, Taketani H, Tsukakoshi Y, Matsushita M, Oda K, Kusumoto K, Kashiwagi Y, Sugiyama M.: A novel transformation system using a bleomycin resistance marker with chemosensitizers for Aspergillus oryzae. Biochem Biophys Res Commun., 383(1), 42-7 (2009).
  • K.-I. Kusumoto, M. Matsushita-Morita, I. Furukawa, S. Suzuki, Y. Yamagata, Y. Koide, H. Ishida, M. Takeuchi and Y. Kashiwagi: Efficient production and partial characterization of aspartyl aminopeptidase from Aspergillus oryzae, Journal of Applied Microbiology, 105, 1711-1719 (2008)
  • K.-I. Kusumoto, I. Furukawa, N. Kitamoto, T. Kimura, M. Matsushita, S. Suzuki, Y.: Survivability of conidia from a recombinant Aspergillus oryzae strain overproducing cellulose, and its parent strain in a soil environment. 食品総合研究所研究報告、72、53-58(2008)
  • 柏木豊、山口加奈子、吉田智子、栗原洋子、木村佳枝、成田清一、楠本憲一、鈴木聡、セルロース担体セル路ファインに対するセルロース結合部位を付加した融合タンパク質の吸着、食品総合研究所研究報告、72、59-64(2008)
  • 迅速ゲノム抽出法及びPCR法を用いた味噌中の真菌ゲノムの検出、鈴木聡、竹谷博子、福岡真里、松下真由美、多田功生、楠本憲一、柏木豊、味噌の科学と技術、55 (5), 311-314 (2007)
  • 楠本憲一、古川育代、鈴木 聡、柏木 豊:麹菌の簡便かつ効率的な胞子形成能の強化法、食品総合研究所研究報告、71、39-43 (2007)
  • Suzuki S, Taketani H, Kusumoto K, Kashiwagi Y.: High-throughput genotyping of filamentous fungus Aspergillus oryzae based on colony direct polymerase chain reaction, Journal of Bioscience and Bioengineering, 102(6), 572-574 (2006)
  • M. Machida, K. Asai, M. Sano, T. Tanaka, T. Kumagai, G. Terai, K. Kusumoto, T. Arima, O. Akita, Y. Kashiwagi , K. Abe, K. Gomi, H. Horiuchi, K. kitamoto, T. Kobayashi, M. Takeuchi, D.W. Denning, J. Galagan, W. Nierman, J. Yu, D. Archer, J. Bennett, D. Bhatnagar, T. Cleaveland, N. Fedrova, O. Gotoh, H. Horikawa, A. Hosoyama, M. Ichinomiya, R. Igarashi, K. Iwashita, P. Juvvadi, M. Kato, Y. Kato, T. Kin, A. Kokubun, H. Maeda, N. Maeyama, J. Maruyama, H. Nagasaki, T. Nakajima, K. Oda, K. Okada, I. Paulsen, K. Sakamoto, T. Sawano, M. Takahashi, K, Takase, Y. Terabayashi, J. Wortman, O. Yamada, Y. Yamagata, H. Anazawa, Y. Hata, Y. Koide, T. Komori, Y. Koyama, T. Minetoki, S. Suharnan, A. Tanaka, K. Isono, S. Kuhara, N. Ogasawara, H. Kikuchi: Genome sequencing and analysis of Aspergillus oryzae, Nature, 438(7071), 1157-1161 (2005)
  • H. Ishida, Y. Hata, A. Kawato, Y. Abe, Y. Kashiwagi: Isolation of a novel promoter for efficient protein production in Aspergillus oryzae, Bioscience, Biotechnology, and Biochemistry, 68 (9), 1849-1857 (2004)
  • 楠本憲一、木村多江、鈴木 聡、柏木 豊: Aspergillus oryzaeのテロメア配列を付加したプラスミドの細胞内における形態の解析、食品総合研究所研究報告、68、43-47(2004)
  • 柏木豊、山口加奈子、栗原洋子、鈴木聡、秋田修、町田雅之、小山泰二、山口庄太郎、五味勝也、阿部敬悦、竹内道雄、小林哲夫、堀内裕之、北本勝ひこ: 麹菌の発現遺伝子の大規模解析、日本醤油研究所雑誌、30 (4)、135-141(2004)
  • 鈴木 聡、竹谷博子、石田博樹、楠本憲一、柏木 豊: Aurintricarboxylic Acid Ammonium Salt (ATA)を用いた米麹からの麹菌全RNA抽出法の検討、食品総合研究所研究報告、68、33-38(2004)
  • H, Akeboshi, Y. Kashiwagi, H. Aoki, T. Tonozuka, A. Nishikawa, Y. Sakano: Construction of an efficient expression system for Aspergillus isopullulanase in Pichia pastoris, and a simple purification method, Bioscience, Biotechnology and Biochemistry, 67 (5), 1149-1153 (2003)
  • Ken-Ichi Kusumoto, Satoshi Suzuki, Yutaka Kashiwagi: Telomeric repeat sequence of Aspergillus oryzae consists of dodeca-nucleotides, Applied Microbiology and Biotechnology, 61 (3), 247-251 (2003)
  • S. Suzuki, H. Taketani, K. Kusumoto, Y. Kashiwagi: High-throughput screening for isolation of enhanced green fluorescent protein expressing transformants of filamentous fungus using an image analyzer, Journal of Bioscience and Bioengineering, 96 (3), 313-316 (2003)
  • 鈴木 聡 楠本憲一 柏木 豊: Construction of a gene trap vector, pPTR-EGFP1, for the filamentous fungus, Aspergillus oryzae、食品総合研究所研究報告、67、33-38(2003)
  • 柏木豊、石田博樹、秦洋二、川戸章嗣、安部康久: アスペルギルス・オリザの新規変異株及び選択マーカー、2004-241170、2004年8月20日出願
  • 柏木豊、楠本憲一、鈴木聡、松下真由美: 麹菌の遺伝子、該麹菌発現遺伝子のプロモーター並びに該プロモーターを利用した外来遺伝子の発現方法、特許第3743803号、2006年2月8日
  • 柏木豊、楠本憲一、鈴木聡: 糸状菌の菌体量測定法、2003-292703、2003年8月13日出願