食品研究部門

微生物機能ユニット

主要成果

論文(2011年以降)

  • Katsuichi Saito, Satoru Tomita, Toshihide Nakamura. (2019) Aggregation of Lactobacillus brevis associated with decrease in pH by glucose fermentation. Bioscience, Biotechnology, and Biochemistry (in press).
  • Toshihide Nakamura, Satoru Tomita, Katsuichi Saito. (2018) Metabolite Profiling in Dough During Fermentation. Food Science and Technology Research, 24: 509-517.
  • Akihito Endo, Fuko Sasaki, Shintaro Maeno, Yu Hamaguchi, Glaezel Angelique, Satoru Tomita, Junichi Nakagawa. (2018) In vitro and in silico characterisation of Lactobacillus paraplantarum D2-1, a starter culture for soymilk fermentation. International Journal of Food Sciences and Nutrition, 69: 857-869.
  • Kazumi Funane, Yukinori Tanaka, Takeshi Hosaka, Kiriko Murakami, Takatsugu Miyazaki, Yuh Shiwa, Shigehachi Gibu, Takashi Inaoka, Ken Kasahara, Nobuyuki Fujita, Hirofumi Yoshikawa, Yoshikazu Hiraga, Kozo Ochi. (2018) Combined Drug Resistance Mutations Substantially Enhance Enzyme Production in Paenibacillus agaridevorans. Journal of Bacteriology, 200: e00188-18.
  • Keitarou Kimura, Takashi Inaoka, Kazutaka Yamamoto. (2018) Metabolome analysis of Escherichia coli ATCC25922 cells treated with high hydrostatic pressure at 400 and 600 MPa. Journal of Bioscience and Bioengineering, 126: 611-616.
  • Yuji Kubo, Supawadee Sriyam, Rikio Nakagawa, Keitarou Kimura. (2018) A Survey of Phage Contamination in Natto-producing Factories and Development of Phage-resistant Bacillus subtilis (natto) Strains. Food Science and Technology Research, 24: 485-492.
  • Nguyen Sy Le Thanh, Keitarou Kimura, Do Thi Tuyen, Le Thi Ngoc Anh. (2018) Isolation, characterization of Bacillus sp. producing heavy metal absorption γ-PGA. Journal of Vietnamese Environment, 9: 49-54.
  • Sy Le Thanh NGUYEN, Takashi INAOKA, Keitarou KIMURA. (2018) Poly-γ-glutamic Acid Production by Bacillus subtilis (natto) under High Salt Conditions. Japan Agricultural Research Quarterly: JARQ, 52: 249-253.
  • Satoru Tomita, Toshihide Nakamura, Sanae Okada. (2018) NMR- and GC/MS-based metabolomic characterization of sunki, an unsalted fermented pickle of turnip leaves. Food Chemistry, 258: 25-34.
  • Satoru Ishihara, Takashi Inaoka, Toshihide Nakamura, Keitarou Kimura, Yasuyo Sekiyama, Satoru Tomita. (2018) Nuclear magnetic resonance- and gas chromatography/mass spectrometry-based metabolomic characterization of water-soluble and volatile compound profiles in cabbage vinegar. Journal of Bioscience and Bioengineering, 126: 53-62.
  • 稲岡隆史、木村啓太郎、中村敏英、冨田理 (2018)ナタデココ生産における酢酸菌のコロニー形態と生産能の相関性、農研機構研究報告食品研究部門、2:21
  • 中村敏英、安藤聡、冨田理(2018)GABA非資化性パン酵母を利用したGABA富化パンの製造、農研機構研究報告食品研究部門、2:47
  • S. Tomita, K. Saito, T. Nakamura, Y. Sekiyama, J. Kikuchi. (2017) Rapid discrimination of strain-dependent fermentation characteristics among Lactobacillus strains by NMR-based metabolomics of fermented vegetable juice. PLOS ONE, 12: e0182229.
  • S. Tomita, S Ikeda, S. Tsuda, N. Someya, K. Asano, J. Kikuchi, E. Chikayama, H. Ono, Y. Sekiyama. (2016) A survey of metabolic changes in potato leaves by NMR-based metabolic profiling in relation to resistance to late blight disease under field conditions. Magnetic Resonance in Chemistry, 55: 120-127.
  • S. Tomita, N. Tanaka, S. Okada. (2017) A rapid NMR-based method for discrimination of strain-specific cell wall teichoic acid structures reveals a third backbone type in Lactobacillus plantarum. FEMS Microbiol. Let. 364: fnx034.
  • T. Inaoka, K. Kimura, K. Morimatsu, K. Yamamoto. (2017) Characterization of high hydrostatic pressure-injured Bacillus subtilis cells. Bioscience, Biotechnology, and Biochemistry, 81: 1235-1240.
  • T. Ozaki, N. Abe, K. Kimura, A. Suzuki, J. Kaneko. (2017) Genomic analysis of Bacillus subtilis lytic bacteriophage φNIT1 capable of obstructing natto fermentation carrying genes for the capsule-lytic soluble enzymes poly-γ-glutamate hydrolase and levanase. Bioscience, Biotechnology, and Biochemistry, 81: 135-146.
  • 木村啓太郎、久保雄司 (2017) 納豆発酵過程での香気成分の変動, 日本食品科学工学会誌, 64: 379-384.
  • K. Kimura, T. Inaoka, K. Yamamoto. (2017) Injury and recovery of Escherichia coli ATCC25922 cells treated by high hydrostatic pressure at 400-600 MPa. Journal of Bioscience and Bioengineering, 123: 698-706.
  • Ando, A. and Nakamura, T. (2016) Prevention of GABA reduction during dough fermentation using a baker's yeast dal81 mutant. Journal of Bioscience and Bioengineering, 122: 441-445.
  • H. Nakao, K. Saito, S. Tomita, Y. Magariyama, Y. Kaizuka, and Y. Takeda. (2016) Direct imaging of carboxymethyl cellulose-mediated aggregation of lactic acid bacteria using dark-field microscopy. Analytical Sciences, 32: 1047-1051.
  • Yano, K., Masuda, K., Akanuma, G., Wada, T., Matsumoto, T., Shiwa, Y., Ishige, T., Yoshikawa, H., Niki, H., Inaoka, T., and Kawamura, F. (2016) Growth and sporulation defects in Bacillus subtilis mutants with a single rrn operon can be suppressed by amplification of the rrn operon. Microbiology, 162: 35-45.
  • 本山志織、ワナシリ・ワナラット、稲岡隆史 (2016) 大腸菌におけるゲノム重複を介した多剤耐性化 食品総合研究所研究報告,No. 80, 87-93.
  • 齋藤勝一、冨田理、中村敏英(2016)Lactobacillus brevisの凝集を引き起こす物質の探索 食品総合研究所研究報告,No. 80, 75-80.
  • S. Tomita, I. C. Lee, I. I. van Swam, S. Boeren, J. Vervoort, P. A. Bron, M. Kleerebezem. (2016) Characterisation of the transcriptional regulation of the tarIJKL-locus involved in ribitol-containing wall teichoic acid biosynthesis in Lactobacillus plantarum. Microbiology, 162, 420-432.
  • H. Oike, A. Aoki-Yoshida, H. Kimoto-Nira, N. Yamagishi, S. Tomita, Y. Sekiyama, M. Wakagi, M. Sakurai, K. Ippoushi, C. Suzuki, and M. Kobori. (2016) Dietary intake of heat-killed Lactococcus lactis H61 delays age-related hearing loss in C57BL/6J mice. Scientific Reports, 6, 23556.
  • M. Kamba, S. Hase, K. Fujii, M. Miyake, K. Sato, K. Kimura, Y. Sakakibara. (2015) Whole-genome sequencing and comparative genome analysis of Bacillus subtilis strains isolated from non-salted fermented soybean foods. PLOS ONE, doi. 10.1371/journal.pone.0141369.
  • Lee, K., Kusumoto, M., Sekizuka, T., Kuroda, M., Uchida, I., Iwata, T., Okamoto, S., Yabe, K., Inaoka, T., and Akiba, M. (2015) Extensive amplification of GI-VII-6, a multidrug resistance genomic island of Salmonella enterica serovar Typhimurium, increase resistance to extended-spectrum cephalosporins. Front. Microbiol. doi: 10.3389/fmicb.2015.00078
  • Wannarat, W., Motoyama, S., Masuda, K., Kawamura, F., and Inaoka, T. (2014) Tetracycline tolerance mediated by gene amplification in Bacillus subtilis. Microbiology 160: 2474-2480.
  • S. Ikeda, T. Takeshi, H. Nakamura, H. Sakai, Y. Usui, T. Okubo, K. Tago, K. Hayashi, Y. Sekiyama, H. Ono, S. Tomita, M. Hayatsu, T. Hasegawa, and K. Minamisawa. (2015) Characterization of leaf blade- and leaf sheath-associated bacterial community and assessment of their responses to environmental changes of CO2, temperature, and nitrogen levels under field conditions. Microbes and Environments, 30, 51-62.
  • Nakamura, T., Yamamoto, M., Saito, K., Ando, A. and Shima, J. (2014) Identification of a gene, FMP21, whose expression levels are involved in thermotolerance in Saccharomyces cerevisiae. AMB Express, 4, 67.
  • Saito, K., Nakamura, T., Kobayashi, I., Ohnishi-Kameyama, M., Ichinosea, H., Kimura, K. and Funane, K. (2014) Xylan-mediated aggregation of Lactobacillus brevis and its relationship with the surface properties and mucin-mediated aggregation of the bacteria. Bioscience, Biotechnology, and Biochemistry, 78(12)2120-2127.
  • Do TH, Nguyen TT, Nguyen TN, Le QG, Nguyen C, Kimura K, and Truong NH. (2014) Mining biomass-degrading genes through Illumina-based de novo sequencing and metagenomic analysis of free-living bacteria in the gut of the lower termite Coptotermes gestroi harvested in Vienam. Journal of Bioscience and Bioengineering, 118 (6), 665-671.
  • 稲岡隆史、草房克江、本山志織 (2014) クロラムフェニコール耐性遺伝子によるゲノム重複を活用した枯草菌育種法 食品総合研究所研究報告,No. 78, 37-41.
  • Shima, Y., Fujisawa, M., Kitagawa, M., Nakano, T., Kimbara, J., Nakamura, N., Shiina, T., Sugiyama, J., Nakamura, T., Kasumi, T. and Ito, Y. (2014) Tomato FRUITFULL homologs regulate fruit ripening via ethylene biosynthesis. Bioscience, Biotechnology, and Biochemistry, 78(2)231-237.
  • T-T. Nguyen, T-H. Do, T-H. Duong, Q-G Le, T-K. Dao, T-T. Nguyen, T-Q. Nguyen, T-T-H. Nguyen, K Kimura, N-H. Truong. (2014) Identification of Vietnamese Coptotermes pest species based on the sequencing of two regions of 16S rRNA gene. Bulletin of Insectology, 67(1), 131-136.
  • S. Tomita, T. Nemoto, Y. Matsuo, T. Shoji, F. Tanaka, H. Nakagawa, H. Ono, J. Kikuchi, M. Ohnishi-Kameyama, and Y. Sekiyama. (2014) A NMR-based, non-targeted multistep metabolic profiling revealed L-rhamnitol as a metabolite that characterised apples from different geographic origins. Food Chemistry, 174, 163-172.
  • Lee, I. I. van Swam, S. Tomita, P. Morsomme, T. Rolain, P. Hols, M. Kleerebezem, and P. A. Bron. (2014) GtfA and GtfB are both required for protein O-glycosylation in Lactobacillus plantarum. Journal of Bacteriology, 196, 1671-1682.
  • Nakamura, T., Yazaki, J., Kishimoto, N., Kikuchi, S., Robertson, A.J., Gusta, L.V. and Ishikawa, M. (2013) Comparison of long-term up-regulated genes during induction of freezing tolerance by cold and ABA in bromegrass cell cultures revealed by microarray analyses. Plant Growth Regulation, 71(2)113-136.
  • 久保雄司、齋藤勝一、ダニエル・ホルヴェック、舟根和美、中川力夫、木村啓太郎 (2013)「菌体外分解酵素が増強された納豆菌rpoB変異株による黒大豆納豆の製造」食品科学工学会誌60 (10), 577-581.
  • Y. Kubo, T. Inaoka, T. Hachiya, M.Miyake, S. Hase, R. Nakagawa, H. Hasegawa, K. Funane, Y. Sakakibara, and K. Kimura. (2013) Development of a rifampicin-resistant Bacillus subtilis strain for natto-fermentation showing enhanced exoenzyme production. Journal of Bioscience and Bioengineering, 115 (6), 654-657.
  • Inaoka, T., and Ochi, K. (2012) Undecaprenyl pyrophosphate involved in susceptibility of Bacillus subtilis to rare earth elements. J. Bacteriol. 194: 5632-5637.
  • S. Tomita, P. de Waard, E. J. Bakx, H. A. Schols, M. Kleerebezem, and P. A. Bron. (2013) The structure of an alternative wall teichoic acid produced by a Lactobacillus plantarum WCFS1 mutant contains a 1,5-linked poly(ribitol phosphate) backbone with 2-α-D-glucosyl substitutions. Carbohydrate Research, 370, 67-71.
  • T. Shiraishi, S. Yokota, N. Morita, S. Fukiya, S. Tomita, N. Tanaka, S. Okada, A. Yokota. (2013) Characterization of lipoteichoic acid from Lactobacillus gasseri JCM 1131T: A novel glycolipid anchor structure. Applied and Environmental Microbiology, 79, 3315-3318.
  • Tanimura, A., Nakamura, T., Watanabe, I., Ogawa, J. and Shima, J. (2012) Isolation of a novel strain of Candida shehatae for ethanol production at elevated temperature. SpringerPlus, 1: 27.
  • Haitani, Y., Tanaka, K., Yamamoto, M., Nakamura, T., Ando, A., Ogawa, J. and Shima, J. (2012) Identification of an acetate-tolerant strain of Saccharomyces cerevisiae and characterization by gene expression analysis. Journal of Bioscience and Bioengineering, 114: 648-651.
  • Watanabe, I., Miyata, N., Ando, A., Shiroma, R., Tokuyasu, K. and Nakamura, T. (2012) Ethanol production by repeated-batch simultaneous saccharification and fermentation (SSF) of alkali-treated rice straw using immobilized Saccharomyces cerevisiae cells. Bioresource Technology, 123: 695-698.
  • Watanabe, I., Ando, A. and Nakamura, T. (2012) Characterization of Candida sp. NY7122, a novel pentose-fermenting soil yeast. Journal of Industrial Microbiology and Biotechnology, 39: 307-315.
  • Fujimoto Z and Kimura K. (2012) Crystal structure of bacteriophage ΦNIT1 zinc peptidase PghP that hydrolyzes γ-glutamyl linkage of bacterial poly-γ-glutamate. Proteins, 80, 722-732.
  • S. Tomita, K. Furihata, N. Tanaka, E. Satoh, T. Nukada, and S. Okada. (2012) Determination of strain-specific wall teichoic acid structures in Lactobacillus plantarum reveals diverse α-D-glucosyl substitutions and high structural uniformity of the repeating units. Microbiology, 158, 2712-2723.
  • P. A. Bron, S. Tomita, I. I. van Swam, D. M. Remus, M. Meijerink, M. Wels, S. Okada, J. M. Wells, and M. Kleerebezem. (2012) Lactobacillus plantarum possesses the capability for wall teichoic acid backbone alditol switching. Microbial Cell Factories, 11, 123.
  • Watanabe, T., Watanabe, I., Yamamoto, M., Ando, A. and Nakamura, T. (2011) A UV-induced mutant of Pichia stipitis with increased ethanol production from xylose and selection of a spontaneous mutant with increased ethanol tolerance. Bioresource Technology, 102: 1844-1848.
  • Kawabata Y, Kimura K, and Funane K. (2011) Extracellular production of cycloisomaltooligosaccharide glucanotransferase and cyclodextran by a protease-deficient Bacillus subtilis host-vector system. Appl. Microbiol. Biotech., 93, 1877-1884.
  • Inaoka, T., and Ochi, K. (2011) Scandium stimulates the production of amylase and bacilysin in Bacillus subtilis. Appl. Environ. Microbiol. 77: 8181-8183.
  • Kubo Y, Rooney AP, Tsukakoshi Y, Nakagawa R, Hasegawa H, and Kimura K. (2011) Phylogenetic analysis of Bacillus subtilis strains applicable to natto (fermented soybean) production. Appl. Environ. Microbiol., 77, 6463-6469.
  • Kimura K, Tran LSP, and Funane K. (2011) Loss of poly-γ-glutamic acid synthesis of Bacillus subtilis (natto) due to IS4Bsu1 translocation to swrA gene. Food Sci. Technol. Res.,17, 447-451.

総説など

  • Keitarou Kimura and Satoshi Yokoyama. (2019) Trends in the application of Bacillus in fermented foods. Current Opinion in Biotechnology, 56: 36-42.

  • 冨田 理 (2019) 木曽の伝統漬物「すんき」と発酵野菜ジュースのメタボローム解析、食糧、第57巻、p15-26.

  • 中村 敏英(2019)パン生地発酵の改良に向けたパン酵母研究、食糧、第57巻、p37-50.

  • 冨田 理 (2018) NMRおよびGC/MSメタボロミクスによる発酵食品成分の包括的解析、日本醸造協会誌、第113巻,11号,p667-678.

  • 山本 和貴, 木村 啓太郎, 稲岡 隆史, 森松 和也, 中浦 嘉子 (2018) 細菌の高圧不活性化における損傷·回復、日本食品科学工学会誌、65:154-162.

  • 中村敏英 (2017)パン酵母の窒素源代謝系因子DAL81の変異によるパン生地発酵過程のGABA消費の低減化、日本醸造協会誌、112:807-811

  • 田中尚人、宮崎 智、鈴木智典、冨田 理、梶川揚申、内野昌孝、五十君靜信、岡田早苗(2017)ユーザーの求める機能性乳酸菌株のオンライン選抜システムの開発、日本微生物資源学会誌、33;31-37

  • 稲岡隆史(2017)、細菌の遺伝子重複による環境適応 -遺伝子の「多コピー化」という細菌のサバイバル戦略-、化学と生物、55:301-302

  • 木村啓太郎(2017)「食と微生物の事典」、 分担執筆「納豆菌」、朝倉書店、pp50-51

  • 中村敏英(2016)酵母を操る乳酸菌:サワー種乳酸菌の多様性 日本乳酸菌学会誌 Vol.27 No.1 pp.53.

  • 関山恭代、池田成志、冨田理 (2015)「農業・食品産業現場におけるNMRメタボロミクスの利用と今後の展望」月刊バイオインダストリー 第32巻 第10号 10頁,シーエムシー出版

  • Shima, J. and Nakamura, T. (2015) Environmental Stresses to Which Yeast Cells Are Exposed During Bioethanol Production from Biomass. In "Stress Biology of Yeasts and Fungi: Applications for Industrial Brewing and Fermentation" ed. by Takagi H., Kitagaki H., Springer Japan, pp.93-106.

  • 冨田理 (2015)「Lactobacillus plantarum菌株間にみられる細胞壁テイコ酸の構造多様性」日本乳酸菌学会誌 Vol.26 No.3 pp.203 (2015)

  • 木村啓太郎 (2015) 納豆菌の発酵能をアップグレード 食糧 53号 pp.49-61

  • 島純、田中晃一、中村敏英(2014)有機酸ストレス耐性酵母の探索・育種とバイオプロセスへの応用 未利用バイオマスの資源化に向けた酵母の機能開拓 化学と生物 Vol.52 No.6 pp.351-353.

  • 中村敏英(2014)パン酵母育種技術の最新動向 JATAFFジャーナル(公益社団法人 農林水産・食品産業技術振興協会)Vol.2 No.12 pp.14-17.

  • 島純、小松崎典子、吉田綾子、安藤聡、中村敏英(2013)パンづくりを支える微生物機能 -酵母と乳酸菌を中心にして- 生物工学会誌 Vol.91, No.11, pp.618-620.

  • 中村敏英(2013)酸耐性酵母の開発と利用 研究ニュース(食品総合研究所) No.30 pp.2-4.

  • 長島實、中村敏英、徳安健、榊原祥清(2013)シリーズ「バイオマス研究の10年を振り返る」10 エタノール変換技術〔1〕バイオマス原料のエタノール発酵と草本系材料の糖化前処理技術 農業および園芸(養賢堂) Vol.88, No.9, pp.961-971.

  • 中村敏英(2013)バイオエタノール生産用ストレス耐性酵母の開発と特性評価 食糧-その科学と技術- 第51号 pp.47-63.

  • 中村敏英(2013)米粉生地の発酵特性評価 低コストで質の良い加工・業務用農産物の安定供給技術の開発 第2分冊(2系 大豆,3系 畑作物,4系 稲,5系 モデルコンソーシアム) 研究成果485 pp.357-360.

  • P. A. Bron, S. Tomita, A. Mercenier, M. Kleerebezem. (2013) Cell surface-associated compounds of probiotic lactobacilli sustain the strain-specificity dogma. Current Opinion in Microbiology, 16, 1-8.

  • Lee, S. Tomita, M. Kleerebezem, and P. A. Bron. (2013) The quest for probiotic effector molecules - Unraveling strain specificity at the molecular level. Pharmacological Research, 69, 61-74.

  • 木村啓太郎 (2012) 納豆研究の新展開-納豆菌とファージの共進化から探る-.生物工学, 6, 315-319.

  • 安藤聡、中村敏英(2011)優れた発酵特性を有する酵母の探索 微生物遺伝資源探索収集調査報告書 Vol.24, pp.51-56.

  • 島純、 安藤聡、中村敏英(2011)環境ストレス耐性に着目したバイオエタノール生産酵母開発の試み 生物工学会誌 Vol.89, No.9, pp.536-538.

  • 木村啓太郎 (2011) 納豆菌と枯草菌の共通点と違い.醸造協会誌,11, 756-762.

  • 木村啓太郎 (2011) 納豆の機能性.「発酵・醸造食品の最新技術と機能性II」, pp.228-232,シーエムシー出版.

  • Inaoka, T., and Ochi, K. (2011) Activation of dormant secondary metabolism neotrehalosadiamine synthesis by RNA polymerase mutation in Bacillus subtilis. Biosci. Biotech. Biochem. 75: 618-623.